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Chapter 4
biology • intermediate 11th

Biological Molecules

Comprehensive notes on Water, Carbohydrates, Proteins, Lipids, Nucleic Acids (DNA/RNA) and enzymes for Chapter 4 Biological Molecules, 11th Class Biology.

Introduction to Biochemistry

Biochemistry: The branch of biology dealing with the chemical components and chemical processes in living organisms.

Chemical Composition of Cell: All living organisms form protoplasm produced from bio-elements. Six main elements (C, H, O, N, P, Ca) make up 99% of the total mass.

Carbon Importance: Carbon is tetravalent, forming stable covalent bonds with other C atoms (catenation) and H, O, N, S, P. It forms the backbone of organic molecules.

Biological Importance of Water

  • Polarity: Water is a polar molecule (dipole). It dissolves polar substances (salts, sugars) becoming a universal solvent for ionic/polar compounds.
  • Hydrogen Bonding: Weak electrostatic attraction between water molecules. Responsible for unique properties like cohesion.
  • High Specific Heat Capacity: Water can absorb/release heat with minimal temperature change (Heat capacity = 1.0 cal/g/°C). This acts as a temperature stabilizer for organisms.
  • Heat of Vaporization: High energy required to convert liquid to gas (574 kcal/kg). Provides cooling effect (evaporation of 2ml water cools 1L body by 1°C).
  • Cohesion & Adhesion: Helps in water transport in plants.
  • Density Anomaly: Ice is less dense than water, floating on top to insulate aquatic life below.

Carbohydrates

Definition: Polyhydroxy aldehydes or ketones, or substances that yield these on hydrolysis. Formula: Cn(H2O)n.

Classification:

  • Monosaccharides: Simple sugars (e.g., Glucose, Fructose). Cannot be hydrolyzed. Classified by carbon count (Triose, Tetrose, Pentose, Hexose).
  • Oligosaccharides: Yield 2-10 monosaccharides on hydrolysis. e.g., Disaccharides like Sucrose (Glucose + Fructose), Lactose (Glucose + Galactose), Maltose (Glucose + Glucose). Linked by Glycosidic Bond.
  • Polysaccharides: Complex, high molecular weight. Yield many monosaccharides. Examples: Starch (Plant storage), Glycogen (Animal storage), Cellulose (Plant cell wall), Chitin (Fungi cell wall).

Lipids

Definition: Heterogeneous group of organic compounds insoluble in water but soluble in organic solvents (ether, alcohol). High energy store (double that of carbs).

Classification:

  • Acylglycerols: Fats and oils. Esters of fatty acids and glycerol. Pure fats are triglycerides.
  • Saturated Fatty Acids: No double bonds (e.g., Palmitic acid). Solid at room temp (Fats).
  • Unsaturated Fatty Acids: Contain double bonds (e.g., Oleic acid). Liquid at room temp (Oils).
  • Phospholipids: Derivatives of phosphatidic acid. Major component of cell membranes. Hydrophobic tail, Hydrophilic head.
  • Waxes: Mixtures of long-chain alkanes and esters. Protective coating.
  • Terpenoids: Made of Isoprenoid units. Include carotenoids, rubber, terpenes.

Proteins

Composition: Polymers of Amino Acids linked by Peptide Bonds (C-N linkage). Most abundant organic compounds in cells (50% dry weight).

Structure Levels:

  • Primary: The sequence of amino acids in a polypeptide chain (determined by DNA). e.g., Insulin sequence (Sanger).
  • Secondary: Coiling or folding due to H-bonds. α-helix (Spiral) and β-pleated sheet (Sheet-like).
  • Tertiary: 3D folding of polypeptide into a globular shape. Stabilized by ionic, H-bonds, disulfide, and hydrophobic interactions. Essential for enzyme function.
  • Quaternary: Association of multiple polypeptide chains (subunits). e.g., Hemoglobin (4 chains: 2 alpha, 2 beta).

Nucleic Acids

Definition: Polymers of Nucleotides. Discovered by F. Miescher (1869). Two types: DNA and RNA.

Nucleotide Structure: Made of Pentose Sugar (Ribose/Deoxyribose) + Nitrogenous Base (Purines A/G, Pyrimidines C/T/U) + Phosphate Group. Linked by Phosphodiester bonds.

Structure of DNA (Watson & Crick Model)

Proposed in 1953:

  • Double Helix: Two polynucleotide strands coiled around each other.
  • Antiparallel: Strands run in opposite directions (5'→3' and 3'→5').
  • Base Pairing: Adenine (A) pairs with Thymine (T) (2 H-bonds); Guanine (G) pairs with Cytosine (C) (3 H-bonds). (Chargaff's Rule: A=T, G=C).
  • Dimensions: Helix diameter = 2nm (20 Å). One full turn = 3.4nm (34 Å) containing 10 base pairs. Distance between adjacent pairs = 0.34nm.

Types of RNA

RNA is single-stranded and contains Uracil (U) instead of Thymine.

  • Messenger RNA (mRNA): Carries genetic code from DNA to ribosome. (3-4% of total RNA).
  • Transfer RNA (tRNA): Transfers amino acids to ribosomes during protein synthesis. (10-20%). Smallest RNA.
  • Ribosomal RNA (rRNA): Major component of ribosomes (80%). Site of protein synthesis.

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