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Chapter 2
biology • intermediate 11th

Bacteria and Viruses

Comprehensive notes, detailed HIV structure, Gram staining mechanism, and exercises for Chapter 2 Bacteria and Viruses, 11th Class Biology (Federal Board 2025).

Bacteria: Discovery and Occurrence

Discovery: Bacteria were first observed by Antonie van Leeuwenhoek in 1673 using a simple self-made microscope. He described them as 'animalcules'. The term 'bacterium' was later coined by Ehrenberg.

Occurrence: They are omnipresent, found in air, water, soil, and inside other organisms. They can survive in extreme environments like hot springs (thermophiles) and acidic environments (acidophiles).

Classification of Bacteria by Shape

  • Cocci (Spherical): Round cells. Can exist as Monococcus (single), Diplococcus (pairs), Streptococcus (chains), or Staphylococcus (clusters like grapes).
  • Bacilli (Rod-shaped): Examples include Escherichia coli and Bacillus subtilis. Can be Diplobacilli or Streptobacilli.
  • Spirilla (Spiral): Spiral or corkscrew-shaped, e.g., Vibrio (comma-shaped) and Spirillum.

Bacterial Cell Structure

  • Flagella: Long, whip-like appendages made of protein 'flagellin' used for motility.
  • Pilli: Short, hollow appendages made of 'pillin' protein. Used for attachment and conjugation (transfer of genetic material).
  • Capsule & Slime: Outer protective layers. Capsule is tightly bound and gummy; Slime is loose and soluble. They protect against phagocytosis.
  • Nucleoid: The region containing the single, circular, double-stranded DNA chromosome. Bacteria lack a true nucleus and nuclear membrane.
  • Plasmids: Small, circular, extra-chromosomal DNA molecules that carry genes for antibiotic resistance and fertility.
  • Mesosomes: Invaginations of the cell membrane involved in respiration and DNA replication (analogous to mitochondria).

Bacterial Cell Wall (Gram Staining)

Composition: Made primarily of Peptidoglycan (murein). Based on Hans Christian Gram's staining technique, bacteria are divided into:

  • Gram-Positive (+ve):
    • Stain Purple/Violet.
    • Have a thick peptidoglycan layer (up to 90% of wall).
    • Contain Teichoic Acids.
    • Lack an outer membrane.
    • Examples: Staphylococcus, Streptococcus.
  • Gram-Negative (-ve):
    • Stain Pink/Red.
    • Have a thin peptidoglycan layer (5-10%).
    • Possess a unique Outer Membrane containing Lipopolysaccharides (LPS).
    • More resistant to antibiotics due to the outer barrier.
    • Examples: E. coli, Salmonella.

Viruses: Characteristics and Structure

Definition: Viruses are non-cellular, obligate intracellular parasites. They contain a genome (DNA or RNA) and protein coat but lack metabolic machinery.

Structure:

  • Core: Genetic material, either DNA or RNA (never both).
  • Capsid: Protein coat surrounding the core, made of subunits called capsomeres. It determines the virus shape.
  • Envelope: Some viruses have an outer lipid membrane derived from the host cell, containing viral glycoproteins (spikes).

Life Cycle of Bacteriophage

Viruses that infect bacteria are called Bacteriophages (e.g., T4 phage).

  • Lytic Cycle (Virulent): The virus commandeers the host machinery to replicate immediately. Steps: Adsorption (Attachment), Penetration (DNA injection), Biosynthesis (Replication), Maturation (Assembly), and Lysis (Bursting of host to release virions).
  • Lysogenic Cycle (Temperate): The viral DNA integrates into the host bacterial chromosome and becomes a Prophage. It replicates passively with the host DNA without destroying the cell. It may spontaneously switch to the Lytic cycle (Induction).

HIV Structure and Life Cycle

HIV (Human Immunodeficiency Virus): Causes AIDS. It is a Retrovirus (RNA virus).

Structure:

  • Envelope: Contains viral glycoproteins gp120 (attachment) and gp41 (fusion).
  • Capsid: Cone-shaped, made of p24 protein.
  • Genome: Two identical strands of single-stranded RNA.
  • Enzymes: Contains Reverse Transcriptase (converts RNA to DNA), Integrase, and Protease.

Life Cycle: Attachment (to CD4 receptors on T-cells) → Fusion → Reverse Transcription (RNA to DNA) → Integration (Viral DNA enters host nucleus) → Transcription & Translation → Assembly → Budding.

Viral Diseases

  • Hepatitis: Inflammation of the liver. Types A, B, C, D, E. Hepatitis B is DNA-based; others are RNA viruses.
  • Herpes: Caused by Herpes Simplex Virus. Types I (Cold sores) and II (Genital).
  • Polio: Caused by Poliovirus. Transmitted via fecal-oral route. Affects the nervous system causing paralysis.
  • Measles & Mumps: Highly contagious Paramixoviruses.
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